icam 5 monoclonal antibodies  (R&D Systems)

Journal: FASEB BioAdvances

Article Title: Endoplasmic Reticulum Stress and Unfolded Protein Response Sensor ERN1 Regulates Organic Dust Induction of Lung Inflammation

doi: 10.1096/fba.2025-00069

Figure Lengend Snippet: Effects of ERN1 chemical inhibitors and ERN1 knockdown on dust extract induction of inflammatory mediator protein levels in Beas2B bronchial epithelial cells. (A–E). Cells were first treated for 1 h with medium alone, 1 μM KIRA6, or 1 μM APY29 prior to incubation with 1% dust extract for 3 h. (F–K). Cells were transfected with 10 nM non‐targeting control siRNA or ERN1 siRNA and treated with 1% dust extract for 24 h. Cellular pro IL1β, ICAM1, and ERN1 protein levels and secreted IL6 and CXCL8 levels were determined by western blotting and ELISA, respectively. Pro IL1β, ICAM1, and ERN1 protein levels were normalized to Actin levels. Representative western blots are shown (A, F). Data shown are mean ± SE ( n = 4–8 for chemical inhibitor experiments and n = 4–5 for siRNA transfection experiments). Statistical significance was analyzed by two‐way ANOVA followed by Sidak's post hoc test for analysis between specific groups (G) and Tukey's post hoc test for analysis between multiple groups (B–E, H–K). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. C, control; DE, dust extract.

Article Snippet: ICAM1 (G‐5) , 1:1000 , sc‐8439, Santa Cruz Biotechnology, Dallas, TX, USA.

Techniques: Knockdown, Incubation, Transfection, Control, Western Blot, Enzyme-linked Immunosorbent Assay